J.-F. Cormier, M. Fortin, J. Fréchette, I. Noiseux, M.L. Vernon, W. Long "The effects of self-absorption and detection geometry on fluorescence intensity and decay lifetime" Proc. of SPIE, 5702:123-134, 2005
Abstract: This paper presents a theoretical model of the effect of the geometry of illumination and collection in fluorescent media, which exhibit self-absorption at sufficiently high concentrations. In order to derive a relation between the incident excitation intensity and the fluorescence emission intensity, we consider the series of paths and transformations that light takes between the source and the detector. The preliminary supporting experiments were conducted on non-turbid liquid fluorescent samples using classical right-angle detection scheme, based on Time-Correlated Single Photon Counting (TCSPC). The fluorescent dyes tested in these experiments (Coumarins 1, 314 and 343) were chosen because they all are excitable at 405 nm, and exhibit varying Stokes shifts. The results suggest that the geometry of the illumination and collection, as well as the self-absorption process, should be taken into account in time-resolved and intensity fluorescence measurements.
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