O. Mermut, P. Gallant, N. Le Bouch, S. Leclair, I. Noiseux, M. L. Vernon, J.-F. Morin, K. Diamond, M. S. Patterson, K. Samkoe, B. Pogue "Magnetic field effects on spectrally resolved lifetime of on-line oxygen monitoring using magneto-optic probes" Proc. of SPIE, 7190, art. no. 71900Y, 2009
Résumé: Multimodal agents that serve as both probes for contrast and light-activated effectors of cellular processes in diseased tissue were developed. These agents were introduced into multicellular tumor spheroids (3D tissue models) and in the chorioallantoic membrane (CAM) of a chicken embryo. The luminescence decay was examined using a novel technique involving a spectrally-resolved fluorescence lifetime apparatus integrated with a weak electromagnet. A spectrallyresolved lifetime setup was used to identify magneto-optic species sensitive to magnetic field effects and distinguish from background emissions. We demonstrate that the applied magnetic fields can alter reaction rates and product distribution of some dyes detected by time- and spectrally-resolved luminescence changes. We will discuss the use of exogenous magneto-optical probes taken up in tumors to both induce phototoxicity, a process that is governed by complex and dynamically evolving mechanisms involving reactive oxygen specie
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